N-硝基-L-精氨酸甲酯對氧驚厥發(fā)作的影響

摘要： 目的:探討一氧化氮合酶(NOS)抑制劑N-硝基-L-精氨酸甲酯(L-NAME)對腦型氧中毒(氧驚厥)發(fā)作的影響。方法:SD大鼠40只,分為氧驚厥組、生理鹽水對照組、L-NAME組和氮-氧混合氣組。L-NAME在高壓氧暴露前10min腹腔注射用藥。觀察各組大鼠氧驚厥始發(fā)時間(ITC)、驚厥嚴重程度(SOC)和存活時間(ST)。結(jié)果:氧驚厥和生理鹽水對照組的ITC、SOC、ST無顯著性差異,應用L-NAME后,ITC、ST明顯延長(P<0.01),SOC顯著下降(P<0.01)。結(jié)論:高壓氧暴露前10min應用L-NAME可延長氧驚厥發(fā)作的潛伏期和生存時間,降低氧驚厥的嚴重程度,具有預防氧驚厥的作用。
Objective: To observe the effects of Nω-Nitro-L-Arginine Methyl ester(L-NAME) on the convulsions in rats suffering from oxygen-induced convulsion. Method: 40 SD rats were divided into oxygen-induced convulsion group, saline control group, nitric oxide synthase inhibitor group and mixed-gas group. L-NAME was used by intraperitoneal injection pre-hyperbaric oxygen exposure. The initial time of convulsion(ITC), severity of convulsion(SOC) and survival time(ST) were observed in each group.Results: There were no significant differences between the ITC、SOC、ST in oxygen-induced convulsion group and saline control group. The ITC、ST in nitric oxide synthase inhibitor group increased significantly compared with that in saline control group,but the SOC decreased significantly.Conclusion: The use of L-NAME pre-hyperbaric oxygen exposure can increase the latent period and the survival time of convulsion in rats suffering from oxygen-induced convulsion , and decrease the severity of convulsion. It indicated that the L-NAME has the preventative effect on the oxygen-induced convulsion.