腹部開放傷合并海水浸泡致急性腎衰犬模型的建立

摘要： 目的:建立腹部開放傷合并海水浸泡致急性腎功能衰竭動(dòng)物模型,為海戰(zhàn)傷的研究提供技術(shù)平臺(tái)。方法:成年雜種犬20只,致腹部開放傷后隨機(jī)分為海水浸泡組和對(duì)照組,每組10只。觀察各時(shí)間點(diǎn)生存率、體溫、血壓、腎功、血?dú)?、滲透壓、病理等變化。結(jié)果:經(jīng)過海水浸泡后,動(dòng)物立即出現(xiàn)低血壓、低溫、高滲,然后逐漸出現(xiàn)代謝性酸中毒,血肌酐和血尿素氮逐漸升高,病理也出現(xiàn)急性腎衰竭表現(xiàn),與對(duì)照組比較有顯著性差別(P<0.05)。結(jié)論:該模型具備戰(zhàn)傷合并海水浸泡的傷情變化特點(diǎn),出現(xiàn)了典型的急性腎衰的病情特點(diǎn),可行且重復(fù)性好,適用于海戰(zhàn)傷致急性腎衰的病理生理和相應(yīng)治療手段的研究。
Objective: To establish a canine model of acute renal failure (ARF) caused by open trauma and seawater immersion that could be used for investigation.Methods:Trauma model was established with twenty grown-up healthy dogs. All dogs were subjected to open abdominal injury,and randomly separated into control group(n=10) and immersion group(n=10). Observe the numerical values of survival rate、 anus temperature、 MAP、 Scr、 BUN、 Osm、 HCO3-、electrolyte and pathologic.Results:After immersion,the dogs presented obvious hypotensive, hypothermia, hyperosmolality,and presented acid-base imbalance eventually.BUN and Scr raised up as time went by and the pathologic showed typical ARF′s change.Conclusions:The model is featured with the typical physiopathologic changes after wound and immersion.and the model is practicable,reasonable and repeatable for investigating the feature of ARF caused by open trauma and seawater immersion.