極低頻脈沖電磁場(chǎng)細(xì)胞生物效應(yīng)及機(jī)制的研究

摘要： 目的研究ELF脈沖電磁波照射細(xì)胞前后細(xì)胞胞漿Ca2+濃度的變化及產(chǎn)生該變化的機(jī)理。方法以實(shí)驗(yàn)為基礎(chǔ),對(duì)細(xì)胞胞漿Ca2+濃度進(jìn)行時(shí)-頻分析,進(jìn)而研究ELF脈沖電磁波在細(xì)胞胞漿Ca2+濃度時(shí)-頻域內(nèi)產(chǎn)生的生物學(xué)效應(yīng)。結(jié)果給出細(xì)胞胞漿Ca2+受ELF脈沖電磁場(chǎng)照射前(a)后(b)的PHMx(t,ν)等值線圖,并結(jié)合液晶生物膜模型,應(yīng)用液晶電流體動(dòng)力不穩(wěn)定的特性闡述了產(chǎn)生該生物效應(yīng)的機(jī)理。結(jié)論細(xì)胞胞漿Ca2+濃度在時(shí)-頻域內(nèi)有其固有的頻譜特征,其中包括連續(xù)譜和離散譜。有的參數(shù)的電磁波可在細(xì)胞胞漿Ca2+濃度的時(shí)-頻域內(nèi)產(chǎn)生生物學(xué)效應(yīng)而有的參數(shù)的電磁波卻不能。若能夠產(chǎn)生生物學(xué)效應(yīng),則有兩個(gè)特征:一是使胞漿Ca2+濃度在時(shí)-頻域內(nèi)的連續(xù)譜變窄了;二是使離散譜的分布和離散譜的頻率發(fā)生了變化。同時(shí),能夠在細(xì)胞胞漿Ca2+濃度的時(shí)-頻域內(nèi)產(chǎn)生生物學(xué)效應(yīng)的電磁波脈沖頻率和脈沖強(qiáng)度是離散和間隔的。
Objective To observe changes of cytosolic Ca2+ levels before and after irradiation by ELF pulsed electromagnetic waves and to study the mechanism of these changes.Methods Basing on the result from experiment,time-frequency analysis was applied to analyze the bio-effects of ELF pulsed electromagnetic field on cytosolic Ca2+ levels.Results The PHMx(t,ν)isoline of cytosolic Ca2+ levels of the cells before(a)and after(b)irradiation by ELF pulsed electromagnetic field was given.Electrohydrodynamic instability and a crystalline liquid biomembrane were applied to explain the mechanism of biological effects on the cells.Conclusion The cytosolic Ca2+ concentration represents a well-defined pattern of spectrum in time-frequency domain,containing continuous and dispersive spectral components.The circs which was considered to induce bio-effects in time-frequency domain presented two unique properties:the continuous spectrum of cellular Ca2+ fluctuations in time-frequency domain was narrowed by the high-energy spectrum component restrained,and the distribution and the frequencies of the dispersive spectra were changed.Furthermore,the pulse frequencies and intensities producing the bio-effects are discrete and distant.